Viral Vector Core

Core Location: 221 EMRB
Phone: 319-335-6726
Director: Patrick Sinn, PhD, Associate Professor, Pediatrics
Phone: 319-335-8190
Office: 6318 PBDB
 Facility Director: Kate Holt, PhD
Phone: 319-335-3467
Office: 200F EMRB


The Viral Vector Core assists researchers, including those in the Holden Comprehensive Cancer Center, who are interested in developing and using viral vector construction. The core utilizes molecular biology techniques to engineer and produce viral and non-viral vectors in quantities necessary for gene transfer in research experiments or preclinical studies. Core staff works closely with investigators to plan and develop gene transfer vectors to fit individual project requirements.

Services Provided

The VVC brings together investigators interested in developing and using, viral and non-viral vectors for gene transfer with specialists in the area of vector construction by:

  • Construct, purify, and perform quality control of:
  • -Recombinant adenovirus
  • -Helper-dependent adenovirus
  • -Adeno-associated virus (AAV)
  • -Lentivirus
  • -Moloney murine retrovirus
  • -Baculovirus
  • -Vaccinia virus
  • Consultations to plan individual project requirements
  • Assist with troubleshooting
  • Design, build, and produce novel vectors
  • Maintain and distribute stocks of recombinant reporter viruses
  • Design efficient RNAi sequences using siSPOTR (
  • Generation of RNAi expression vectors
  • Cloning expression cassettes into vector shuttles