Gene Transfer Vector Core

Director: Beverly L. Davidson, PhD, Associate Professor
Phone: 319-335-6726
FAX: 319-353-5572
Office: 221 EMRB

Purpose

The Gene Vector Core assists researchers interested in developing and using viral vector construction. The core utilizes molecular biology techniques to engineer and produce viral and non-viral vectors in quantities necessary for gene transfer in research experiments or preclinical studies. Core staff works closely with investigators to plan and develop gene transfer vectors to fit individual project requirements.

Services Provided

The University of Iowa Carver College of Medicine GTVC brings together investigators interested in developing and using, viral and non-viral vectors for gene transfer with specialists in the area of vector construction by:

  • Consultation with the Principal Investigator to plan and develop transfer vectors to fit individual projects requirements.
  • Assist investigators with troubleshooting existing projects.
  • Design and development of novel vectors.
  • Develop novel methods of virus production such as the RapAdTM System for adenovirus production.
  • Generation of RNAi expression vectors.
  • Purify, concentrate, and perform quality control on preparations of recombinant adenovirus, adeno-associated virus (AAV), moloney murine retrovirus, lentivirus, baculovirus, and vaccinia virus.
  • Maintain and distribute standard cell lines and stocks of recombinant reporter viruses.