Vitamin B12 level
The vitamin B12 level is a blood test that measures how much vitamin B12 is in your blood.
How the Test is Performed
A blood sample is needed.
How to Prepare for the Test
You should not eat or drink for about 6 to 8 hours before the test.
Certain medicines may affect the results of this test. Your doctor will tell you if you need to stop taking any medicines. Do not stop any medicine before talking to your doctor.
Medicines that can affect the test result include:
- Para-aminosalicylic acid
How the Test will Feel
When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or a slight bruise. This soon goes away.
Why the Test is Performed
This test is most often done when other blood test tests suggest a condition called megaloblastic anemia. Pernicious anemia is a form of megaloblastic anemia caused by poor vitamin B12 absorption. This can occur when the stomach makes less of the substance the body needs to properly absorb vitamin B12.
Your doctor may also order a vitamin B12 test if you have certain nervous system symptoms. A low level of B12 can cause numbness or tingling in the arms and legs, weakness, and loss of balance.
Other conditions for which the test may be done:
- Sudden severe confusion (delirium)
- Loss of brain function (dementia)
- Dementia due to metabolic causes
Normal values are 200 - 900 picograms per milliliter (pg/mL).
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or may test different samples. Talk to your doctor about what your specific test results mean.
What Abnormal Results Mean
Values of less than 200 pg/mL are a sign of a vitamin B12 deficiency. People with this deficiency are likely to have or develop symptoms. Older adults with vitamin B12 levels between 200 and 500 pg/mL may also have symptoms.
Causes of vitamin B12 deficiency include:
- Not enough vitamin B12 in diet (rare, except with a strict vegetarian diet)
- Diseases that cause malabsorption (for example, celiac disease and Crohn disease)
- Lack of intrinsic factor, a protein that helps the intestine absorb vitamin B12
- Above normal heat production (for example, with hyperthyroidism)
A Schilling test can find the cause of a vitamin B12 deficiency.
An increased vitamin B12 level is uncommon. Usually, excess vitamin B12 is removed in the urine.
Conditions that can increase B12 level include:
- Liver disease (such as cirrhosis or hepatitis)
- Myeloproliferative disorders (for example, polycythemia vera and chronic myelocytic leukemia)
There is very little risk involved with having your blood taken. Veins and arteries vary in size from one patient to another and from one side of the body to the other. Taking blood from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight, but may include:
- Excessive bleeding
- Fainting or feeling light-headed
- Hematoma (blood accumulating under the skin)
- Infection (a slight risk any time the skin is broken)
Mason JB. Vitamins, trace minerals, and other micronutrients. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine. 24th ed. Philadelphia, Pa: Elsevier Saunders; 2011:chap 225.
Salwen MJ. Vitamins and trace elements. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, Pa: Elsevier Saunders; 2011:chap 26.
Last reviewed 2/24/2014 by Todd Gersten, MD, Hematology/Oncology, Florida Cancer Specialists & Research Institute, Wellington, FL. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.
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